Use of a redox probe for an electrochemical RNA–ligand binding assay in microliter droplets

Abstract : In this work, we report an affordable, sensitive, fast and user-friendly electroanalytical method for monitoring the binding between unlabeled RNA and small compounds in microliter-size droplets using a redox-probe and disposable miniaturized screen-printed electrochemical cells. Increasing evidence has shown that non-coding RNAs play a key role in many biological functions and are involved not only in infectious but also in many other human diseases. 1 Despite an exploding number of biological studies suggesting RNA-regulated pathways as potential drug targets, the design of small, drug-like compounds able to selectively bind and modulate RNA functions is still difficult. 2 This is quite surprising if one considers that interaction with ribosomal RNA is one of the major modes of action of antibiotics 3 and that small metabolites are known to regulate basic functions of prokaryotes by interacting with riboswitches. 4
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H. Guyon, François Mavré, Marjorie Catala, Serge Turcaud, Franck Brachet, et al.. Use of a redox probe for an electrochemical RNA–ligand binding assay in microliter droplets. Chemical Communications, Royal Society of Chemistry, 2017, 53 (6), pp.1140-1143. ⟨10.1039/c6cc07785d⟩. ⟨hal-02185366⟩

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