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Dynamics of single-stranded DNA migration in denaturing polyacrylamide slab-gel electrophoresis

Abstract : We describe an original apparatus for the study of the dynamics of single stranded DNA migration. Four detectors based on laser-induced fluorescence (LIF) are equidistantly placed on one migration lane, allowing repeated measurements of the same DNA band at different positions along migration. This article presents the characteristics and performances of this system and focuses on the data analysis, showing how the multiple detection scheme enables the study of band broadening and band resolution during a migration run. Our results suggest the existence of anomalous (nontherma
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http://hal.in2p3.fr/in2p3-00010607
Contributor : Suzanne Robert Connect in order to contact the contributor
Submitted on : Tuesday, November 13, 2001 - 11:43:35 AM
Last modification on : Wednesday, September 16, 2020 - 4:01:47 PM

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  • HAL Id : in2p3-00010607, version 1

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Z. Djouadi, S. Bottani, M-A. Duval, R. Siebert, H. Tricoire, et al.. Dynamics of single-stranded DNA migration in denaturing polyacrylamide slab-gel electrophoresis. Electrophoresis, Wiley-VCH Verlag, 2001, 22, pp.3527-3532. ⟨in2p3-00010607⟩

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