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$^{Cf}$252-plasma desorption mass spectrometry of unmodified lipid A:Fragmentation patterns and localization of fatty acids

Abstract : The fragmentation patterns of synthetic Escherichia coli-type lipid A in plasma desorption mass spectrometry (PDMS) in both negative- and positive-ion modes were determined. Negative-ion spectra gave signals for the main diphosphorylated (intact) molecular species in their native proportions. Intact and alkaline-treated lipid A in this mode gave, for the glucosamine I moiety, easily identified signals that have not been previously reported in PDMS, These spectra gave enough information to localize the fatty acids. The procedure was verified with relatively homogeneous lipids A prepared from Salmonella minnesota R595 and Neisseria meningitidis lipopolysaccharides, and then applied to the previously unstudied Yersinia entercolitica O:11,24 lipid A to obtain the localization of its fatty acids. The possibility of obtaining this much information from two negative-ion spectra was attributed to the method, described earlier, of preparing the samples.In the positive-ion mode, about half of the E. coli ions containing diglucosamine appeared as monodephosphorylated species and/or as Na adducts. The intact glucosamine II moiety and its fragment ions gave signals none of which were Na adducts. With lipids A prepared from S. minnesota, N. meningitidis, and Y. enterocolitica, similar fragmentation patterns were observed, For lipid A structure determination, the positive-ion mode could play a confirmatory role, The above results and some of those reported by others were compared.
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http://hal.in2p3.fr/in2p3-00015696
Contributor : Suzanne Robert <>
Submitted on : Tuesday, May 23, 2000 - 12:30:09 PM
Last modification on : Thursday, November 19, 2020 - 2:20:21 PM

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D. Karibian, A. Brunelle, L. Aussel, M. Caroff. $^{Cf}$252-plasma desorption mass spectrometry of unmodified lipid A:Fragmentation patterns and localization of fatty acids. Rapid Communications in Mass Spectrometry, Wiley, 1999, 13, pp.2252-2259. ⟨in2p3-00015696⟩

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