Study of the interaction between europium (III) and Bacillus subtilis: fixation sites, biosorption modeling and reversibility

Abstract : In order to elucidate the underlying mechanisms involved in the biosorption of metal ions, potentiometric titrations, complexation studies, and time-resolved laser-induced fluorescence spectroscopy (TRLFS) measurements were used to characterize the interaction between Eu(III) and Bacillus subtilis. The reversibility of the interaction between Eu(III) and Bacillus subtilis was studied by a cation-exchange technique using the Chelex resin. For complexation studies in the presence of 0.15 mol/l of NaCl, the metal ion, the biomass, concentrations and the pH were varied. The adsorption data were quantified by a surface complexation model without electrostatic term. The data on the Eu(III)/B.subtilis system at pH 5 were satisfactorily described by one site at which Eu(III) was bound through one carboxylic function of the bacteria. With increasing pH, another site should be considered, involving a phosphate-bound environment. This was partially confirmed by time-resolved laser-induced fluorescence spectroscopy. In addition to this, it was evidenced that the site availability was dependent on the nature of the cation, i.e., a proton or Eu(III). Finally, it was shown that, at pH 5, the Eu(III)/Bacillus subtilis equilibrium was reversible.
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http://hal.in2p3.fr/in2p3-00025906
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Submitted on : Friday, April 7, 2006 - 10:59:06 AM
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Sandrine Markai, Yves Andres, Gilles Montavon, Bernd Grambow. Study of the interaction between europium (III) and Bacillus subtilis: fixation sites, biosorption modeling and reversibility. Journal of Colloid and Interface Science, Elsevier, 2003, 262, pp.351-361. ⟨10.1016/S0021-9797(03)00096-1⟩. ⟨in2p3-00025906⟩

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