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Physiological importance of the connective tissue in the human amnion. Role of magnesium.

Abstract : The elemental ionic distribution in the epithelial layer (EL) and in connective tissue (CT = compact layer + fibroblast layer) of the human amniotic membrane has been studied in reference samples, after conservation in a physiological fluid (Hanks' solution) and after addition of 2 mM MgCl2 in Hanks' solution. Particle induced X-ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological fluid, with regard to reference samples, the monovalent ions (Na+, K +, Cl-) concentrations were identical on both layers. This data indicates that the connective tissue, in particular the compact layer, acts as a buffer which fix minerals. Mg2+ and Ca2+ levels were higher in EL than in CT. The addition of MgCl2 in Hanks' solution induced a decrease of the monovalent ion concentrations in both layers except Na+ level in EL which remained constant, an increase of the Mg2+ level in both layers, while the Ca2+ remained constant. These data indicate the possible role of connective tissue in pregnancies complicated by poly or oligohydramnios.
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Contributor : Pascale Chambon <>
Submitted on : Wednesday, October 4, 2006 - 3:04:49 PM
Last modification on : Monday, May 6, 2019 - 11:50:22 AM


  • HAL Id : in2p3-00103477, version 1


M. Bara, P. Moretto, J. Durlach, A. Guiet-Bara. Physiological importance of the connective tissue in the human amnion. Role of magnesium.. Magnesium Research, John Libbey Eurotext, 2003, 16, pp.35-42. ⟨in2p3-00103477⟩



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