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An interdisciplinary approach to investigate the impact of cobalt in a human keratinocyte cell line

Abstract : Since in nuclear power plants, risks of skin contact contamination by radiocobalt are significant, we focused on the impact of cobalt on a human cutaneous cell line, i.e. HaCaT keratinocytes. The present paper reports an interdisciplinary approach aimed at clarifying the biochemical mechanisms of metabolism and toxicity of cobalt in HaCaT cells. Firstly, a brief overview of the used instrumental techniques is reported. The following parts present description and discussion of results concerning: (i) toxicological studies concerning cobalt impact towards HaCaT cells (ii) structural and speciation fundamental studies of cobalt-bioligand systems, through X-ray absorption spectroscopy (XAS), ab initio and thermodynamic modelling (iii) preliminary results regarding intracellular cobalt speciation in HaCaT cells using size exclusion chromatrography/inductively coupled plasma-atomic emission spectroscopy (SEC/ICP-AES) and direct in situ analysis by ion beam micropobe analytical techniques.
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http://hal.in2p3.fr/in2p3-00129377
Contributor : Pascale Chambon <>
Submitted on : Wednesday, February 7, 2007 - 10:54:24 AM
Last modification on : Saturday, March 6, 2021 - 3:11:42 AM

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C. Bresson, C. Lamouroux, C. Sandre, M. Tabarant, N. Gault, et al.. An interdisciplinary approach to investigate the impact of cobalt in a human keratinocyte cell line. Biochimie, Elsevier, 2006, 88 (11), pp.1619-1629. ⟨10.1016/j.biochi.2006.09.003⟩. ⟨in2p3-00129377⟩

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