Skip to Main content Skip to Navigation
Journal articles

In vivo 212Pb/212Bi generator using indium-DTPA-tagged liposomes

Abstract : Indium-DTPA-tagged liposomes were studied in the present work as carriers of in vivo 212Pb / 212Bi generator to be used in targeted alpha therapy. The liposomal uptake of 212Pb, into preformed liposomes, was investigated using different lipophilic chelate (DCP, 2,3-dimercapto-1-propanol (BAL), sodium acetate, and A23187), as a function of various parameters (temperature, concentrations of lipids, Pb, DTPA,...) with 212Pb as a tracer. Different formulations of liposomes were tested to evaluate the radiolabeling efficiency. No complexing agent was necessary for the passage of Pb2+ through the membrane. It occurs naturally via a partial permeability of the lipid bilayer which increases with the temperature. A complexing agent (DTPA) appears necessary to concentrate Pb in the internal compartment of the liposomes. Conditions were found (T = 65°C, internal DTPA concentration of 0.025 M, pH 7.4, ...) yielding a high and rapid uptake of 212Pb in liposomes. The protocol established provides a novel method for the efficient entrapment of about 2-3 Pb atoms per liposome with a yield of 75% in conditions relevant for nuclear medicine.
Document type :
Journal articles
Complete list of metadatas

Cited literature [13 references]  Display  Hide  Download

http://hal.in2p3.fr/in2p3-00617427
Contributor : Gilles Montavon <>
Submitted on : Monday, August 29, 2011 - 9:26:03 AM
Last modification on : Friday, June 19, 2020 - 3:08:40 AM
Long-term archiving on: : Wednesday, November 30, 2011 - 2:20:33 AM

Files

article-version_finale.pdf
Files produced by the author(s)

Identifiers

Citation

A. Le Du, M. Mougin-Degraef, Eliot Patrick Botosoa, Aurore Rauscher, Alain Faivre-Chauvet, et al.. In vivo 212Pb/212Bi generator using indium-DTPA-tagged liposomes. Radiochimica Acta, R Oldenbourg Verlag GMBH, 2011, 99, pp.743-749. ⟨10.1524/ract.2011.1871⟩. ⟨in2p3-00617427⟩

Share

Metrics

Record views

909

Files downloads

977