R. Ortega 1
1 IPCV - Interface Physique et Chimie pour le Vivant
CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
Abstract : The knowledge of the intracellular distribution of biological relevant metals is important to understand their mechanisms of action in cells, either for physiological, toxicological or pathological processes. However, the direct detection of trace metals in single cells is a challenging task that requires sophisticated analytical developments. The combination of micro-PIXE with RBS and STIM (Scanning Transmission Ion Microscopy) allows the quantitative determination of trace metal content within sub-cellular compartments. The application of STIM analysis provides high spatial resolution imaging (< 200 nm) and excellent mass sensitivity (< 0.1 ng). Application of the STIM-PIXE-RBS methodology is absolutely needed when organic mass loss appears during PIXE-RBS irradiation. This combination of STIM-PIXE-RBS provides fully quantitative determination of trace element content, expressed in μg/g, which is a quite unique capability for micro-PIXE compared to other micro-analytical methods such as the electron and synchrotron x-ray fluorescence. Examples of micro-PIXE studies for sub-cellular imaging of trace elements in various fields of interest will be presented: in patho-physiology of trace elements involved in neurodegenerative diseases such as Parkinson's disease, and in toxicology of metals such as cobalt. Read More: http://www.worldscientific.com/doi/abs/10.1142/S0129083512400220
Mots-clés : PIXE cell trace element
Type de document :
Article dans une revue
International Journal of PIXE, World Scientific Publishing, 2012, 22, pp.51-56. <10.1142/S0129083512400220>
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Soumis le : vendredi 7 décembre 2012 - 09:16:03
Dernière modification le : lundi 13 octobre 2014 - 15:43:25




R. Ortega. MICRO-PIXE FOR SINGLE CELL ANALYSIS. International Journal of PIXE, World Scientific Publishing, 2012, 22, pp.51-56. <10.1142/S0129083512400220>. <in2p3-00762336>



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