Skip to Main content Skip to Navigation
Journal articles

Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

Abstract : The present study describes hybrid nanoparticles, built by alternate deposition of siRNA and modified polyethyleneimine (tyrosine-grafted PEI or tyrosine/galactose-grafted PEI) on calcium phosphate nanoparticles. These "easy to produce" nanoparticles (NPs) present an efficient gene silencing effect demonstrated in vitro in a luciferase expressing cell culture model and in vivo in a tumour xenograft mouse model. The luciferase gene silencing percentage reached up to 95% in vitro with biocompatible doses of siRNA. Interestingly, we show by SPECT imaging of radiolabeled particles that without modifying the size, stability and in vitro efficiency, the grafting of a sugar moiety on PEI can modify the in vivo biodistribution of the particles. The proof of concept that galactose-grafting on PEI could change biodistribution without changing the gene silencing efficiency makes them versatile tools for specific delivery of small interfering RNA. As they have been designed so far, biodistribution is mainly located in the liver and thus these innovative nanoparticles open a realistic and feasible strategy for siRNA delivery into the liver in vivo.
Document type :
Journal articles
Complete list of metadatas

http://hal.in2p3.fr/in2p3-00936774
Contributor : Brigitte Gaillard <>
Submitted on : Monday, January 27, 2014 - 2:11:04 PM
Last modification on : Thursday, November 5, 2020 - 11:50:34 AM

Identifiers

Collections

Citation

Thirunavukkarasu Devarasu, Roxane Saad, Ali Ouadi, Benoit Frisch, Eric Robinet, et al.. Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors. Journal of materials chemistry‎ B, Royal Society of Chemistry, 2013, 1, pp.4692-4700. ⟨10.1039/C3TB20557F⟩. ⟨in2p3-00936774⟩

Share

Metrics

Record views

567