158 articles – 2185 Notices  [english version]
HAL : inserm-00662829, version 1

Fiche détaillée  Récupérer au format
Journal of Structural Biology 177, 2 (2012) 239-47
Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.
Ewelina Kosior1, Sylvain Bohic1, 2, Heikki Suhonen1, Richard Ortega3, Guillaume Devès3, Asuncion Carmona3, Florence Marchi4, 5, Jean Francois Guillet5, Peter Cloetens1

Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.
1 :  ESRF - European Synchrotron Radiation Facility
2 :  GIN - U836 - Grenoble Institut des Neurosciences
3 :  CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
4 :  Physics department - UFR PHITEM
5 :  Surface Science Laboratory
INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
Sciences du Vivant/Ingénierie biomédicale/Médecine nucléaire
X-ray fluorescence – phase contrast imaging – synchrotron – trace elements
Liste des fichiers attachés à ce document : 
Kosior_JSB-2011_Combined_use_of_hard.pdf(495.6 KB)