Toward non-linear endomicroscopy for exploration of the pulmonary airways : preliminary spectroscopic study of human lung tissue using monomode fiber

Donald Peyrot; Darine Abi Haidar; Claire Lefort; Nicolas Sandeau; Tigran Mansuryan; Frédéric Louradour; Sergei Kruglik; Geneviève Bourg-Heckly

HAL: hal-00688280, version 1

DOI: 10.1117/12.875440

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Endoscopic Microscopy VI, Nonlinear Endomicroscopy, San franscisco - Californie : États-Unis (2011)

Toward non-linear endomicroscopy for exploration of the pulmonary airways : preliminary spectroscopic study of human lung tissue using monomode fiber

Donald Peyrot¹, Darine Abi Haidar², Claire Lefort³, Nicolas Sandeau, Tigran Mansuryan³, Frédéric Louradour³, Sergei Kruglik, Geneviève Bourg-Heckly

(2011)

We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.

1:	LOA - Laboratoire d'optique appliquée

2:	IMNC - Imagerie et Modélisation en Neurobiologie et Cancérologie

3:	XLIM - XLIM

Research team: PHOTONIQUE


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From: Véronique Maury <>
Submitted on: Tuesday, 17 April 2012 11:37:23
Updated on: Tuesday, 17 April 2012 11:47:02