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Progress in Solid State Chemistry 34 (2006) 257-266
Novel phosphate–phosphonate hybrid nanomaterials applied to biology
Bruno Bujoli1, Hélène Roussière1, Gilles Montavon2, Samia Laïb1, Pascal Janvier1, Bruno Alonso3, Franck Fayon3, Marc Petit1, Dominique Massiot3, Jean-Michel Bouler4, Jérome Guicheux4, Olivier Gauthier4, Sarah M. Lane5, Guillaume Nonglaton1, Muriel Pipelier1, Jean Léger6, Daniel R. Talham5, Charles Tellier7

A new process for preparing oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. On the other hand, the reaction of a bisphosphonate bone resorption inhibitor (Zoledronate) with calcium deficient apatites (CDAs) was studied as a potential route to local drug delivery systems active against bone resorption disorders. A simple mathematical model of the Zoledronate/CDA interaction was designed that correctly described the adsorption of Zoledronate onto CDAs. The resulting Zoledronate-loaded materials were found to release the drug in different phosphate-containing media, with a satisfactory agreement between experimental data and the values predicted from the model.
1 :  LSOHOOM - Laboratoire de Synthèse Organique (Hétérochimie organique, organoéléments et matériaux)
2 :  SUBATECH - Laboratoire SUBATECH Nantes
3 :  CRMHT - Centre de recherches sur les matériaux à haute température
4 :  LIOAD - Laboratoire d'ingénierie osteo-articulaire et dentaire
5 :  DEPARTMENT OF CHEMISTRY - Department of Chemistry, University of Florida
6 :  Physiopathologie et pharmacologie cellulaires et moléculaires
7 :  U3B - Unité de Biotechnologie, Biocatalyse et Biorégulation

Sciences du Vivant/Toxicologie
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