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Rat interleukin-1 beta binding sites in rat hypothalamus and pituitary gland.
Marquette C., Van Dam A.-M., Ban E., Lanièce P., Crumeyrolle-Arias M. et al
Neuroendocrinology 62, 4 (1995) 362-9 - http://hal.archives-ouvertes.fr/hal-00405261
Sciences du Vivant/Neurosciences/Neurobiologie
Rat interleukin-1 beta binding sites in rat hypothalamus and pituitary gland.
Christel Marquette1, 2, 3, Anne-Marie Van Dam4, Elisabeth Ban2, Philippe Lanièce5, Michèle Crumeyrolle-Arias2, Gilles Fillion2, Frank Berkenbosch4, France Haour2
1 :  LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux
CNRS : UMR5249 – CEA : DSV/IRTSV – Université Joseph Fourier - Grenoble I
17, rue des Martyrs 38054 GRENOBLE CEDEX 9
2 :  Pharmacologie Neuro-Immuno-Endocrinienne
Institut Pasteur de Paris
25-28 rue du Dr Roux 75015 Paris
3 :  Rage
Institut Pasteur de Paris
25-28 rue du Dr Roux
4 :  Research Institute Neurosciences
Free University, Amsterdam
Free University, Medical Faculty, Department of Pharmacology, Amsterdam
5 :  IMNC - Imagerie et Modélisation en Neurobiologie et Cancérologie
CNRS : UMR8165 – IN2P3 – Université Paris XI - Paris Sud – Université Paris VII - Paris Diderot
BATIMENT 104 15 Rue Georges Clémenceau 91406 ORSAY CEDEX
In this study, radiolabeled recombinant rat interleukin-1 beta (r125I-IL-1 beta) was used to localize and characterize IL-1 beta binding in rat hypothalamus and pituitary gland by quantitative autoradiography. The ability of this ligand to bind to type I IL-1 receptor was first tested on murine lymphoma cells (EL-4). In the rat-tissue sections, high densities of specific r125I-IL-1 beta binding sites were localized in the anterior as well as the posterior pituitary and in the choroid plexus. A fine labeling was observed in meninges and third ventricle walls while no binding was detected in the hypothalamic nuclei. Saturation experiments, in the anterior and posterior pituitary, revealed one specific binding site with an affinity constant (Kd) of 0.5 nM. Competition experiments were achieved using either rat IL-1 beta (rIL-1 beta) or human IL-1s (hIL-1 alpha, hIL-1 beta and IL-1 receptor antagonist: hIL-1a). Affinity constants (Ki) were drastically different according to the ligand used, while Ki values were found similar in anterior and posterior pituitary. Competition with rIL-1 beta revealed one binding affinity (Ki of 0.1 nM range). In contrast, competition with hIL-1 beta revealed two binding affinities: a high (Ki: 0.1 pM range) and a low one (Ki: 1 nM range). Competition with hIL-1ra was obtained for high concentrations only (Ki: 10-100 nM range), whereas human IL-1 alpha (hIL-1 alpha) was unable to compete at 1-100 nM.(ABSTRACT TRUNCATED AT 250 WORDS)

Neuroendocrinology (Neuroendocrinology)
Publisher Karger
ISSN 0028-3835 (eISSN : 1423-0194)
Articles dans des revues avec comité de lecture

Animals – Autoradiography – Binding Sites – Dose-Response Relationship – Drug – Hypothalamus – Interleukin-1 – Male – Pituitary Gland – Radioligand Assay – Rats – Wistar