version française rss feed
HAL : hal-00425221, version 1

Fiche concise  Récupérer au format
Metabolic profiling strategy of Caenorhabditis elegans by whole-organism nuclear magnetic resonance.
Blaise B. J., Giacomotto J., Triba M. N., Toulhoat P., Piotto M. et al
Journal of Proteome Research 8, 5 (2009) 2542-50 - http://hal.archives-ouvertes.fr/hal-00425221
Sciences du Vivant/Biochimie, Biologie Moléculaire/Biologie moléculaire
Metabolic profiling strategy of Caenorhabditis elegans by whole-organism nuclear magnetic resonance.
Benjamin J Blaise, Jean Giacomotto1, Mohamed N Triba2, Pierre Toulhoat3, Martial Piotto4, Lyndon Emsley5, Laurent Ségalat1, Marc-Emmanuel Dumas6, Bénédicte Elena7
1 :  CGMC - Centre de génétique moléculaire et cellulaire
CNRS : UMR5534 – Université Claude Bernard - Lyon I
bat. 741 43 Bvd du 11 Novembre 1918 69622 VILLEURBANNE CEDEX
2 :  PCMMB - Physico-chimie moléculaire des membranes biologiques
CNRS : UMR7099 – Université Paris VII - Paris Diderot
13 Rue Pierre et Marie Curie 75005 PARIS
3 :  IPNL - Institut de Physique Nucléaire de Lyon
CNRS : UMR5822 – IN2P3 – Université Claude Bernard - Lyon I
4 :  Bruker Biospin
34 rue de l'Industrie 67166 Wissembourg
5 :  LC - Laboratoire de Chimie
CNRS : UMR5182 – École Normale Supérieure (ENS) - Lyon
Ens de Lyon, Site Jacques Monod - 46 allée d'Italie - 69364 Lyon cedex 07
6 :  NETEC - Neuroépidémiologie Tropicale et Comparée
Université de Limoges : EA3174 – IFR145 GEIST – Institut d'Epidémiologie Neurologique et de Neurologie Tropicale
IENT - Faculté de Médecine 2, rue du Docteur marcland 87025 LIMOGES cedex
7 :  IBCP - Institut de biologie et chimie des protéines [Lyon]
CNRS : UMR5086 – Université Claude Bernard - Lyon I
7 Passage du Vercors 69367 LYON CEDEX 07
In this study, we present a methodology for metabotyping of C. elegans using 1H high resolution magic angle spinning (HRMAS) whole-organism nuclear magnetic resonance (NMR). We demonstrate and characterize the robustness of our metabolic phenotyping method, discriminating wild-type N2 from mutant sod-1(tm776) animals, with the latter being an otherwise silent mutation, and we identify and quantify several confounding effects to establish guidelines to ensure optimal quality of the raw data across time and space. We monitor the sample stability under experimental conditions and examine variations arising from effects that can potentially confuse the biological interpretation or prevent the automation of the protocol, including sample culture (breeding of the worms by two biologists), sample preparation (freezing), NMR acquisition (acquisition by different spectroscopists, acquisition in different facilities), and the effect of the age of the animals. When working with intact model organisms, some of these exogenous effects are shown to be significant and therefore require control through experimental design and sample randomization.

Journal of Proteome Research (J Proteome Res)
Publisher American Chemical Society
ISSN 1535-3893 (eISSN : 1535-3907)
Articles dans des revues avec comité de lecture

Animals – Caenorhabditis elegans – Magnetic Resonance Spectroscopy – Metabolome – Metabolomics – Models – Biological – Mutation