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Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies.
David P., Viollon C., Alexandre E., Azimzadeh A., Nicod L. et al
Human & Experimental Toxicology / Human and Experimental Toxicology 17, 10 (1998) 544-553 - http://hal.archives-ouvertes.fr/hal-00502782
Sciences du Vivant/Sciences pharmaceutiques
Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies.
P. David1, C. Viollon, E. Alexandre, A. Azimzadeh, L. Nicod2, P. Wolf3, D. Jaeck, K. Boudjema, L. Richert4
1 :  LPNHE - Laboratoire de Physique Nucléaire et de Hautes Énergies
http://www-lpnhep.in2p3.fr
CNRS : UMR7585 – IN2P3 – Université Pierre et Marie Curie (UPMC) - Paris VI – Université Paris VII - Paris Diderot
Barre 12-22, 1er étage 4 Place Jussieu 75252 Paris Cedex 05
France
2 :  Ingénierie et biologie cellulaire et tissulaire
http://ifr133-ibct.univ-fcomte.fr/index.htm
INSERM : IFR133 – CHU Besançon – Université de Franche-Comté – EFS
1, Boulevard Alexandre Fleming 25020 Besancon Cedex
France
3 :  SYRTE - Systèmes de Référence Temps Espace
http://syrte.obspm.fr/
CNRS : UMR8630 – INSU – Observatoire de Paris – Université Pierre et Marie Curie (UPMC) - Paris VI
61 Av de l'Observatoire 75014 PARIS
France
4 :  Optimisation Cellulaire et Métabolique
Université de Franche-Comté
France
A new isolating procedure of human hepatocytes has been developed using two-step collagenase digestion by a non-perfusion procedure (NP) of non-wedge liver biopsies. 1. A yield of 2-7 x 10(6) hepatocytes/g liver, 52-95% viability and 13-75% attachment were obtained from liver biopsies weighing 6-60 g, comparable to that obtained when using the classical perfusion procedure (P) to isolate human hepatocytes from wedge liver samples of 50-150 g. 2. In culture, human hepatocytes obtained by NP remained attached to plastic for up to 5 days and displayed the usual morphological characteristics. Their metabolic capacities, assessed by liver-specific albumin and urea synthesis and by CYP-dependent and conjugation pathways, were equivalent to those of human hepatocytes obtained by P. In addition, they responded adequately to specific CYP inducers, demonstrating that they constitute a model in which human drug metabolism and toxicity studies can be performed.
Anglais

Human & Experimental Toxicology / Human and Experimental Toxicology
internationale
Articles dans des revues avec comité de lecture
10/1998
17
10
544-553

Adult – Aged – 80 and over – Albumins – Biopsy – Blotting – Western – Carcinoma – Hepatocellular – Cell Adhesion – Cell Separation – Cell Survival – Collagenases – Cytochrome P-450 Enzyme System – Enzyme Induction – Female – Glutathione – Glutathione Transferase – Humans – Liver Neoplasms – Male – Middle Aged – Tumor Cells – Cultured – Urea