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Titanium dioxide nanoparticles induced intracellular calcium homeostasis modification in primary human keratinocytes. Towards an in vitro explanation of titanium dioxide nanoparticles toxicity.
Simon M., BARBERET P., Delville M.-H., Moretto P., Seznec H.
Nanotoxicology 5, 2 (2011) 125-139 - http://hal.archives-ouvertes.fr/hal-00598703
Chimie/Matériaux
Titanium dioxide nanoparticles induced intracellular calcium homeostasis modification in primary human keratinocytes. Towards an in vitro explanation of titanium dioxide nanoparticles toxicity.
Marina Simon1, Philippe BARBERET1, Marie-Hélène Delville2, Philippe Moretto1, Hervé Seznec1
1 :  CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
http://www.cenbg.in2p3.fr/
CNRS : UMR5797 – IN2P3 – Université Sciences et Technologies - Bordeaux I
Chemin du Solarium - BP 120 - 33175 Gradignan Cedex
France
2 :  ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux
http://www.icmcb-bordeaux.cnrs.fr/
CNRS : UPR9048 – Université de Bordeaux – Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)
87 Av du Dr A. Schweitzer 33608 PESSAC CEDEX
France
Abstract Deciphering the molecular basis of toxicology mechanism induced by nanoparticles (NPs) remains an essential challenge. Ion Beam Analysis (IBA) was applied in combination with Transmission Electron Microscopy and Confocal Microscopy to analyze human keratinocytes exposed to TiO(2)-NPs. Investigating chemical elemental distributions using IBA gives rise to a fine quantification of the TiO(2)-NPs uptake within a cell and to the determination of the intracellular chemical modifications after TiO(2)-NPs internalization. In addition, fluorescent dye-modified TiO(2)-NPs have been synthesized to allow their detection, precise quantification and tracking in vitro. The internalization of these TiO(2)-NPs altered the calcium homeostasis and induced a decrease in cell proliferation associated with an early keratinocyte differentiation, without any indication of cell death. Additionally, the relation between the surface chemistry of the TiO(2)-NPs and their in vitro toxicity is clearly established and emphasizes the importance of the calcium homeostasis alteration in response to the presence of TiO(2)-NPs.
Anglais

Nanotoxicology
Publisher Informa Healthcare
ISSN 1743-5390 (eISSN : 1743-5404)
internationale
Articles dans des revues avec comité de lecture
2011
5
2
125-139

11080