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Nanotoxicology 2 (2008) 218-231
Is there penetration of titania nanoparticles in sunscreens through skin? A comparative electron and ion microscopy study
E. Gontier, M. Ynsa, T. Biacuteroacute, J. Hunyadi, B. Kiss, K. Gaacutespaacuter, T. Pinheiro, J. Silva, P. Filipe, J. Stachura, W. Dabros, T. Reinert, P. Moretto1, T. Butz, J. Surlegraveve-Bazeille
(04/12/2008)

We report on a comparative study by Transmission Electron Microscopy (HRTEM) and Scanning Transmission Ion Microscopy (STIM) combined with Rutherford Backscattering Spectrometry (RBS) and Particle Induced X-Ray Emission (PIXE) on ultra-thin and thin cross-sections, respectively, of various skin samples (porcine skin, healthy human skin, human skin grafted on a severe combined immuno-deficient mouse model) to which we applied topically various formulations containing titanium dioxide (TiO2) nanoparticles with primary particle sizes in the range from 20-100 nm. Whereas the HRTEM and STIM/PIXE images reveal clear differences - mainly related to the different thickness of the cross-sections - they unambiguously show that penetration of TiO2 nanoparticles is restricted to the topmost 3-5 corneocyte layers of the stratum corneum (SC).
1 :  CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
Sciences du Vivant/Toxicologie

Physique/Physique/Biophysique

Sciences du Vivant/Ecologie, Environnement