Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology

C. Habchi; D.T. Nguyen; Ph. Barberet; S. Incerti; Ph. Moretto; A. Sakellariou; H. Seznec

HAL : in2p3-00426833, version 1

DOI : 10.1016/j.nimb.2009.03.070

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Nuclear Instruments and Methods in Physics Research Research 267 (2009) 2107-2112

Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology

C. Habchi¹, D.T. Nguyen, Ph. Barberet¹, S. Incerti¹, Ph. Moretto¹, A. Sakellariou, H. Seznec¹

(15/06/2009)

The DISRA (Discrete Image Space Reconstruction Algorithm) reconstruction code, created by A. Sakellariou, was conceived for the ideal case of complete three-dimensional (3D) PIXET (Particle Induced Xray Emission Tomography) data. This implies two major difficulties for biological samples: first, the long duration of such experiments and second, the subsequent damage that occurs on such fragile specimens. For this reason, the DISRA code was extended at CENBG in order to probe isolated PIXET slices, taking into account the sample structure and mass density provided by 3D STIMT (Scanning Transmission Ion Microscopy Tomography) in the volume of interest. This modified version was tested on a phantom sample and first results on human cancer cells are also presented.

1 :	CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan

Thème(s)

Physique/Physique/Biophysique

Physique/Physique/Instrumentations et Détecteurs

Sciences du Vivant/Ingénierie biomédicale/Imagerie

Mot(s)-clé(s) : Scanning Transmission Ion Microscopy Particle Induced X-ray Emission Computed tomography Micro-tomography 3D imaging Non-destructive characterisation Nuclear microprobe Human cell


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Contributeur : Virginie Mas <>
Déposé pour le compte de : Claire Habchi <>
Soumis le : Jeudi 29 Octobre 2009, 14:45:04
Dernière modification le : Jeudi 29 Octobre 2009, 15:13:11