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Characterization of At- species in simple and biological media by high performance anion exchange chromatography coupled to gamma detector.
Sabatié-Gogova A., Champion J., Huclier S., Michel N., Pottier F. et al
Analytica Chimica Acta 721 (2012) 182 - http://hal.in2p3.fr/in2p3-00681129
Sciences du Vivant/Biotechnologies
Sciences du Vivant/Ingénierie biomédicale/Médecine nucléaire
Characterization of At- species in simple and biological media by high performance anion exchange chromatography coupled to gamma detector.
A. Sabatié-Gogova ()1, J. Champion1, S. Huclier1, N. Michel ()1, F. Pottier ()1, N. Galland ()2, Z. Asfari ()3, M. Chérel4, Gilles Montavon ()1
1 :  SUBATECH - Laboratoire SUBATECH Nantes
CNRS : UMR6457 – IN2P3 – Université de Nantes – École Nationale Supérieure des Mines - Nantes
4, rue Alfred Kastler - 44070 Nantes Cedex 03
2 :  CEISAM - Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation
Université de Nantes – CNRS : UMR6230
UFR des Sciences et des Techniques - 2 rue de la Houssiniere BP 92208 - 44322 NANTES Cedex 3
3 :  IPHC - Institut Pluridisciplinaire Hubert Curien
CNRS : UMR7178 – Université de Strasbourg
4 :  CRCNA - Centre de Recherche en Cancérologie Nantes - Angers
INSERM : U892 – CNRS : UMR6299 – Hôtel-Dieu de Nantes – Université de Nantes
1 Place Alexis-Ricordeau, 44000 Nantes
Astatine is a rare radioelement belonging to the halogen group. Considering the trace amounts of astatine produced in cyclotrons, its chemistry cannot be evaluated by spectroscopic tools. Analytical tools, provided that they are coupled with a radioactive detection system, may be an alternative way to study its chemistry. In this research work, High Performance Anion Exchange Chromatography (HPAEC) coupled to a gamma detector (γ) was used to evaluate astatine species under reducing conditions. Also, to strengthen the reliability of the experiments, a quantitative analysis using a reactive transport model has been done. The results confirm the existence of one species bearing one negative charge in the pH range 27.5. With respect to the other halogens, its behavior indicates the existence of negative ion, astatide At-. The methodology was successfully applied to the speciation of the astatine in human serum. Under fixed experimental conditions (pH 7.47.5 and redox potential of 250 mV) astatine exists mainly as astatide At- and does not interact with the major serum components. Also, the method might be useful for the in vitro stability assessment of 211At-labelled molecules potentially applicable in nuclear medicine.

Articles dans des revues avec comité de lecture
Analytica Chimica Acta (Anal Chim Acta)
Publisher Elsevier Masson
ISSN 0003-2670 

Astatine Speciation Ion-exchange Human serum
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