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Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.
Kosior E., Bohic S., Suhonen H., Ortega R., Devès G. et al
Journal of Structural Biology 177, 2 (2012) 239-47 - http://www.hal.inserm.fr/inserm-00662829
Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.
Ewelina Kosior1, Sylvain Bohic1, 2, Heikki Suhonen1, Richard Ortega3, Guillaume Devès3, Asuncion Carmona3, Florence Marchi ()4, 5, Jean Francois Guillet5, Peter Cloetens ()1
1 :  ESRF - European Synchrotron Radiation Facility
http://www.esrf.fr/
ESRF
6 rue Jules Horowitz BP220 38043 GRENOBLE CEDEX
France
2 :  GIN - U836 - Grenoble Institut des Neurosciences
http://neurosciences.ujf-grenoble.fr/
INSERM : U836 – Université Joseph Fourier - Grenoble I – CHU Grenoble – CEA : DSV/IRTSV
UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9
France
3 :  CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
http://www.cenbg.in2p3.fr/
CNRS : UMR5797 – IN2P3 – Université Sciences et Technologies - Bordeaux I
Chemin du Solarium - BP 120 - 33175 Gradignan Cedex
France
4 :  Physics department - UFR PHITEM
Université Joseph Fourier - Grenoble I
Grenoble
France
5 :  Surface Science Laboratory
European Synchroton Radiation Facility (ESRF)
Grenoble
France
INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
CP
Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.
Sciences du Vivant/Ingénierie biomédicale/Médecine nucléaire
Anglais
1047-8477

Journal of Structural Biology (J Struct Biol)
Publisher Elsevier
ISSN 1047-8477 (eISSN : 1095-8657)
internationale
Articles dans des revues avec comité de lecture
02/2012
11/12/2011
177
2
239-47

X-ray fluorescence – phase contrast imaging – synchrotron – trace elements
Algorithms – Animals – Calibration – Cell Nucleus – Microscopy – Fluorescence – Phase-Contrast – PC12 Cells – Particle Size – Potassium – Rats – Reference Standards – Single-Cell Analysis – Trace Elements – X-Rays – Zinc
12487
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