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Journal of Chromatography B 878, 28 (2010) 2826-2830
Experimental studies of OH degrees radical/pressure dependence of arginase activity using a molecular chromatography approach
C. André1, F. Ibrahim2, T. Gharbi3, G. Herlem3, Y.-C. Guillaume1

Arginase is an enzyme which plays a role in pathophysiology such as hypertension. Here we demonstrated for the first time the direct implication of pressure and OH degrees radical formation on the arginase activity via a novel analytical procedure. Pressure increased arginase activity in the range 12-52 bars. Activation by OH degrees radical showed a hyperbolic response. The OH degrees radicals produced were significantly inhibited by sulfasalazine (SAZ) and the inhibition of OH degrees radicals parallels the inhibition of arginase activity.
1 :  Laboratoire de Chimie Analytique (EA4267)
2 :  IPNO - Institut de Physique Nucléaire d'Orsay
3 :  FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies
Sciences de l'ingénieur/Optique / photonique