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Biomaterials 27 (2006) 3195-3200
Influence of the zinc concentration of zinc substituted hydroxyapatite on cytokines production by human monocytes in vitro
Alexia Grandjean Laquerriere1, Patrice Laquerriere2, 3, Edouard Jallot4, Jean-Marie Nedelec5, Terry M. Phillips1

A possible complication associated with the implantation of HA-based prosthesis is the release of particles. Those particles can be phagocyted by monocytes that are among the first cells to colonize the inflammatory site. The activated monocytes produce inflammatory mediators such as cytokines that cause osteoclasts activation. The present work studies the effect of zinc-substituted hydroxyapatite particles with various concentrations of zinc on cytokines production (TNF-α, IL-1β, IL-6, IL-10, and IL-8) by human monocytes LPS-stimulated or not. The concentrations used were 0.5%, 1%, 2%, and 5%. Our data demonstrates that the zinc has an effect on cytokines production. It decreases the production of TNF- α and increases the production of IL-8 by unstimulated cells. Using LPS-stimulated cells, it decreases the production of inflammatory cytokines and increases the production of anti-inflammatory cytokine (IL-10). So zinc-substituted hydroxyapatite has favourable effects on the cytokines production by monocytes.
1 :  Ultramicro Analytical Immunochemistry Resource,
2 :  Interfaces biomatériaux/tissus hôtes
3 :  Supramolecular Structure and Function Resource,
4 :  LPC - Laboratoire de Physique Corpusculaire [Clermont-Ferrand]
5 :  LMI - Laboratoire des Matériaux Inorganiques
Hydroxyapatite particles – Cytokines – Inflammation – Zinc – Monocytes
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