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Human & Experimental Toxicology / Human and Experimental Toxicology 17, 10 (1998) 544-553
Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies.
P. David1, C. Viollon, E. Alexandre, A. Azimzadeh, L. Nicod2, P. Wolf3, D. Jaeck, K. Boudjema, L. Richert4
(10/1998)

A new isolating procedure of human hepatocytes has been developed using two-step collagenase digestion by a non-perfusion procedure (NP) of non-wedge liver biopsies. 1. A yield of 2-7 x 10(6) hepatocytes/g liver, 52-95% viability and 13-75% attachment were obtained from liver biopsies weighing 6-60 g, comparable to that obtained when using the classical perfusion procedure (P) to isolate human hepatocytes from wedge liver samples of 50-150 g. 2. In culture, human hepatocytes obtained by NP remained attached to plastic for up to 5 days and displayed the usual morphological characteristics. Their metabolic capacities, assessed by liver-specific albumin and urea synthesis and by CYP-dependent and conjugation pathways, were equivalent to those of human hepatocytes obtained by P. In addition, they responded adequately to specific CYP inducers, demonstrating that they constitute a model in which human drug metabolism and toxicity studies can be performed.
1 :  LPNHE - Laboratoire de Physique Nucléaire et de Hautes Énergies
2 :  Ingénierie et biologie cellulaire et tissulaire
3 :  SYRTE - Systèmes de Référence Temps Espace
4 :  Optimisation Cellulaire et Métabolique
Sciences du Vivant/Sciences pharmaceutiques