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| Titre : |
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Cellular in vivo imaging reveals coordinated regulation of pituitary microcirculation and GH cell network function. |
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| Auteur(s) : |
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Chrystel Lafont1, Michel G Desarménien1, Mathieu Cassou1, François Molino1, Jérôme Lecoq2, David Hodson1, Alain Lacampagne3, Gerard Mennessier ( )4, Taoufik El Yandouzi1, Danielle Carmignac, Pierre Fontanaud1, Helen Christian, Nathalie Coutry1, Marta Fernandez-Fuente, Serge Charpak2, Paul Le Tissier, Iain C A F Robinson, Patrice Mollard1 |
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| Résumé : |
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Growth hormone (GH) exerts its actions via coordinated pulsatile secretion from a GH cell network into the bloodstream. Practically nothing is known about how the network receives its inputs in vivo and releases hormones into pituitary capillaries to shape GH pulses. Here we have developed in vivo approaches to measure local blood flow, oxygen partial pressure, and cell activity at single-cell resolution in mouse pituitary glands in situ. When secretagogue (GHRH) distribution was modeled with fluorescent markers injected into either the bloodstream or the nearby intercapillary space, a restricted distribution gradient evolved within the pituitary parenchyma. Injection of GHRH led to stimulation of both GH cell network activities and GH secretion, which was temporally associated with increases in blood flow rates and oxygen supply by capillaries, as well as oxygen consumption. Moreover, we observed a time-limiting step for hormone output at the perivascular level; macromolecules injected into the extracellular parenchyma moved rapidly to the perivascular space, but were then cleared more slowly in a size-dependent manner into capillary blood. Our findings suggest that GH pulse generation is not simply a GH cell network response, but is shaped by a tissue microenvironment context involving a functional association between the GH cell network activity and fluid microcirculation. |
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Langue du texte
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Anglais |
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| Journal : |
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Proceedings of the National Academy of Sciences of the United States of America |
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non spécifiée |
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| Type de publication : |
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Articles dans des revues avec comité de lecture |
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| Date de publication : |
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02/03/2010 |
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107 |
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| Numéro : |
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9 |
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| Page, identifiant, ... : |
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4465-70 |
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| Descripteur(s) MeSH : |
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Animals – Growth Hormone: metabolism – Male – Mice – Inbred C57BL – Transgenic – Microcirculation – Pituitary Gland: blood supply – cytology – metabolism |
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| Référence interne : |
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LPTA:10-045 |
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