The Bacterial Replicative Helicase Loader DciA is a DNA Condenser - Institut Gustave Roussy Access content directly
Preprints, Working Papers, ... Year : 2023

The Bacterial Replicative Helicase Loader DciA is a DNA Condenser

Sonia Baconnais
Magali Noiray
Magali Aumont-Nicaise
Emil Gp Stender
Romain Le Bars
Eric Le Cam

Abstract

Abstract The loading of the bacterial replicative helicase is an essential step for genome replication and depends on the assistance of accessory proteins. Several of these proteins have been identified across the bacterial phyla. DciA is the most common loading protein in bacteria, yet the one whose mechanism is the least understood. We have previously shown that Vc DciA from Vibrio cholerae, composed of a globular KH-like domain followed by an unfolded extension, has a strong affinity for DNA. Here, we characterized the droplets formed by Vc DciA upon interaction with a short single-stranded substrate. We demonstrate the fluidity of these droplets using light microscopy and address their network organization through electron microscopy, thereby bridging events to conclude on a liquid-liquid phase separation behavior. Additionally, we observe the recruitment of Vc DnaB inside the Vc DciA-DNA droplets. We show that DnaC from Escherichia coli is also competent to form these condensate structures in the presence of ssDNA. Our data open up possibilities for the involvement of DciA in the formation of non-membrane compartments within the bacterium, facilitating the assembly of replication players with the chromosomal DNA.
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hal-04265049 , version 1 (30-10-2023)

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Stéphanie Marsin, Sylvain Jeannin, Sonia Baconnais, Hélène Walbott, Gérard Pehau-Arnaudet, et al.. The Bacterial Replicative Helicase Loader DciA is a DNA Condenser. 2023. ⟨hal-04265049⟩
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