Surface characterization of biological nanodomains using NP-ToF-SIMS - IN2P3 - Institut national de physique nucléaire et de physique des particules Access content directly
Journal Articles Surface and Interface Analysis Year : 2013

Surface characterization of biological nanodomains using NP-ToF-SIMS

F.A. Fernandez-Lima
  • Function : Author
J.D. Debord
  • Function : Author
E. A. Schweikert
  • Function : Author
K. A. Kellersberger
  • Function : Author
M. Smotherman
  • Function : Author

Abstract

This paper describes the application of nanoparticle bombardment with time-of-flight secondary ion mass spectrometry (NP-ToF-SIMS) for the analysis of native biological surfaces for the case of sagittal sections of mammalian brain tissue. The use of high energy, single nanoparticle impacts (e.g. 520 keV Au400) permits desorption of intact lipid molecular ions, with enhanced molecular ion yield and reduced fragmentation. When coupled with complementary molecular ion fragmentation and exact mass measurement analysis, high energy nanoparticle probes (e.g. 520 keV Au400 NP) provide a powerful tool for the analysis of the lipid components from native brain sections without the need for surface preparation and with ultimate spatial resolution limited to the desorption volume per impact (~103 nm3).

Dates and versions

in2p3-00859671 , version 1 (09-09-2013)

Identifiers

Cite

F.A. Fernandez-Lima, J.D. Debord, E. A. Schweikert, S. Della-Negra, K. A. Kellersberger, et al.. Surface characterization of biological nanodomains using NP-ToF-SIMS. Surface and Interface Analysis, 2013, 45, pp.294-297. ⟨10.1002/sia.4901⟩. ⟨in2p3-00859671⟩
19 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More