The International Conference on B-Physics at Frontier Machines, “Beauty 2016”, was held in the Hotel Mercure Vieux-Port, Marseille, France from 2nd to 6th May 2016. It was the 16th edition of the series “International Conference on B-Physics at Frontier Machines”, initiated in the Czech Republic in 1993. The aims of the conference are to review the latest theoretical and experimental results in heavy flavour physics and to discuss the future programmes. Beauty 2016 covered a wide range of topics including studies of CP violation and rare decay properties of beauty and charm hadrons. The conference attracted 91 scientists from all over the world, with a programme consisting of 66 invited talks split into 18 theoretical and 48 experimental presentations. In addition, nine early career researchers presented posters with short plenary talks in a dedicated session.