In this study we evaluate the dynamical collisional energy loss of heavy quarks, their interaction rate as well as the different transport coefficients (drag and diffusion coefficients, $\hat{q}$, etc). We calculate these different quantities for i) perturbative partons (on-shell particles in the vacuum with fixed and running coupling) and ii) for dynamical quasi-particles (off-shell particles in the QGP medium at finite temperature $T$ with a running coupling in temperature as described by the dynamical quasi-particles model). We use the perturbative elastic $(q(g) Q \rightarrow q (g) Q)$ cross section for the first case, and the Infrared Enhanced Hard Thermal Loop cross sections for the second. The results obtained in this work demonstrate the effects of a finite parton mass and width on the heavy quark transport properties and provide the basic ingredients for an explicit study of the microscopic dynamics of heavy flavors in the QGP - as formed in relativistic heavy-ion collisions - within transport approaches developed previously by the authors.